Heterogeneity of Autoantibodies to Factor VIII : Differences in Specificity for Apparently Distinct Antigenic Determinants of Factor
نویسنده
چکیده
The interference of antibodies to factor VIII coagulant protein (Vlll:C) of 9 nonhemophilic patients with the binding to factor VIII coagulant antigen (VIII:CAg) of a reference hemophilic ‘251-Fab’ reagent, used in a liquid phase Vlll:CAg assay, was studied. The binding competition was estimated from immunoradiometric assay (IRMA) doseresponse slope of Vlll:CAg present in patient plasma. interference of antibodies with the ‘251-Fab’ binding to Vlll:CAg in normal plasma, and the displacement of antibody from the complexes with VlIl:CAg by the 1251 Fab’. Antibody populations from three patients were studied in detail; in the VIII:CAg assay. two of them interfered with the ‘251-Fab’ binding. and one did not (patient 1 ). The
منابع مشابه
Heterogeneity of autoantibodies to factor VIII: differences in specificity for apparently distinct antigenic determinants of factor VIII coagulant protein.
The interference of antibodies to factor VIII coagulant protein (VIII:C) of 9 nonhemophilic patients with the binding to factor VIII coagulant antigen (VIII:CAg) of a reference hemophilic 125I-Fab' reagent, used in a liquid phase VIII:CAg assay, was studied. The binding competition was estimated from immunoradiometric assay (IRMA) dose-response slope of VIII:CAg present in patient plasma, inter...
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